Study on the antifungal effect and mycolytic activity of the biocontrol agent Chaetomium subaffine LB-1

The antifungal effect and mucolytic activity of a newly screened biocontrol strain Chaetomium subaffine LB-1 were researched in this study. results found that has good effects on the test plant pathogenic fungi Botrytis cinerea Pers. ex Fr., Fusarium oxysporum f. sp. cucumerinum Owen Alternaria solani (Ellis & Martin) Sorauer dual culture assay, with inhibition rates 61.39, 50.34 51.22%, respectively. Flocculated dissolved hyphae phytopathogenic observed at interaction zone dual-cultured PDA plate, but normal. cell-free filtrate significant inhibitory three tested poison plate assay; colonies, vesiculated flocculated also plates supplemented filtrate. Clear halo zones around colonies protease pectinase cellulose indicating could produce enzymes protease, cellulase. However, activities chitinase β-l,3-glucase not detected their from LB-1. An obvious oil-displaced circle was formed oil spreading test, surface-active substance might be contained These proved agent C. exert its via living competition mycolysis, latter may obtained by production mycolytic substances. .